In neuroscience and genetics research, scientists often need to simultaneously capture multi-angle fluorescent signals and locomotion behavior of model organisms (such as nematodes). However, traditional imaging methods are limited by the tradeoff between exposure time, signal intensity, and optical noise, making it difficult to balance image clarity and signal fidelity:

Contradiction between motion blur and weak fluorescence:
Nematodes have high movement speeds (50–200 μm/s). If exposure is extended to enhance fluorescence brightness, motion streaks greater than 10 μm can occur, which severely affects trajectory analysis.

Signal attenuation and crosstalk in spectral imaging:
Multi-angle light splitting drastically reduces effective light intensity. In addition, excitation light scattering further lowers the signal-to-noise ratio, interfering with both quantitative neuron fluorescence analysis and behavior association studies.