Researchers working in neuroscience and genetics often need to record multi-angle fluorescence signals from model organisms such as C. elegans, while also tracking their movement. Traditional imaging systems struggle to keep both clarity and accuracy because of limits in exposure time, low signal levels, and optical noise.

Motion blur vs. weak fluorescence
C. elegans move at 50–200 μm/s. Longer exposure times brighten fluorescence but introduce motion blur greater than 10 μm, making it difficult to analyze movement and behavior.

Signal loss and interference in multi-angle spectral imaging
Splitting light across multiple angles reduces usable signal strength. Scattered excitation light lowers the signal-to-noise ratio, affecting quantitative fluorescence studies and neuron-behavior analysis.